Comparative Analysis of Orthologous Mast Cell Chymases Active Sites Using Bioinformatics Tools

Mast cells, neutrophils, basophils, natural killer and cytotoxic T cells are among the hematopoietic cell lines originating from immune cells. The importance of mammalian mast in innate immunity has piqued scientific community's interest. cytoplasmic granules Histamine, proteoglycans, proteins, cytokines examples compounds (mediators) that produce granules. When external stimuli received, these released, causing degranulation. generate a lot proteases including chymase, tryptase, type A carboxypeptidase, other things. structure chymase cma1 was unknown mice, but it shares 74 percent its human sequence with CMA1, which used as reference. CMA1 to establish interpret mouse structure. Significant residues active site, such catalytic triads binding residues, were examined. position triad been discovered be similar. Val 175 197, respectively, replaced two Ala 192 Gly 214 site target, according residue analysis. We may deduce this substitution cleavage specificity varies chymase.